This tech could revolutionize surgery and neuroscience research. The Microimager's compact, minimally invasive design opens new possibilities for precision-guided brain surgery, high-resolution monitoring of brain diseases, and real-time imaging in living animals.
PITTSBURGH -- Scientists have created a camera so small it's thinner than a human hair, flexible enough to bend with your movements, and capable of seeing deep inside living brain tissue without causing major damage. Researchers at Carnegie Mellon University developed this mini machine to capture high-resolution images of brain activity in real time.
The device, called the "Microimager," measures just 7 micrometers thick and 400 micrometers wide. That's roughly 14 times thinner than a human hair. Unlike bulky traditional endoscopes that can severely damage delicate brain tissue, this ultra-thin camera can slip between neurons, according to the research published in Biomedical Optics Express.
Surgeons could one day use this technology to precisely identify tumor boundaries during operations, potentially saving more healthy brain tissue. Researchers studying neurological diseases like Alzheimer's or Parkinson's could observe brain cells in action without the invasive procedures currently required. And unlike rigid fiber optic cameras that can only capture a single point of light, this flexible device has 20 independent channels that create detailed images.
How This Microscopic Camera Actually Works
Traditional endoscopes, the snake-like cameras doctors thread through the body during procedures, are relatively massive compared to this new invention. Even the thinnest commercial fiber used in brain imaging has a diameter of 200 micrometers, making it over 11 times larger than the Microimager.
The researchers built their camera using something called "Parylene photonics," essentially tiny light highways made from biocompatible polymers that the body won't reject. These microscopic waveguides act like fiber optic cables, but they're flexible enough to bend and twist without breaking.
Each waveguide captures light from fluorescent molecules in brain tissue. The device then channels this light through its 20 separate pathways to create a pixelated image, similar to how your smartphone camera combines millions of tiny sensors to create a photo.
The researchers embedded tiny 45-degree mirrors at both ends of each waveguide. These mirrors redirect light vertically, allowing the camera to capture images along its thin profile rather than just at its tip.
To prove their device actually works, the research team conducted several experiments. First, they tested whether the camera could distinguish between different patterns by placing an optical mask with bright and dark stripes over the input end. The Microimager successfully resolved stripes as small as 30 micrometers wide, roughly one-third the width of a human hair.
But the real test came with living tissue. The researchers used brain slices from genetically modified mice whose neurons were engineered to glow green when active. When they placed these tissue samples over their microscopic camera, it successfully captured the fluorescent signals and relayed them to an external detector.
The device could also detect differences in depth. When fluorescent beads were placed at different heights above the camera, one at the surface and another 70 micrometers away, the device accurately showed the closer bead as brighter than the more distant one. This three-dimensional sensing capability could be crucial for surgeons trying to understand the exact location of important structures.
In their most ambitious test, the researchers demonstrated that their camera could capture brain activity in real time. They electrically stimulated brain tissue while monitoring it with the Microimager, successfully detecting the calcium flashes that occur when neurons fire. The correlation between input and output signals was 0.6, proving the device could track the rapid changes that occur during brain function.
Making the Impossible Possible
Creating such a tiny, functional camera required solving several engineering problems that had been topics of discussion with researchers for years. One major breakthrough came from rethinking how to manufacture the device. Previous attempts used metal masks during fabrication, which created rough edges that scattered light and reduced image quality. The Carnegie Mellon team switched to using smooth photoresist masks, resulting in much cleaner waveguides.
They also had to figure out how to make the device both incredibly thin and durable enough for medical use. The solution involved layering different biocompatible materials: Parylene C forms the core of each waveguide, while PDMS (a silicone-based polymer) provides the cladding. This combination creates a significant difference in how light bends through each material, effectively trapping light within each waveguide channel.
The researchers measured light loss through their waveguides at 8.48 decibels per centimeter, essentially meaning the device efficiently transmits light without major signal degradation. For comparison, this performance rivals much larger conventional optical devices.
While the current device requires an external light source and detector, the researchers envision a fully integrated system where some waveguides deliver excitation light while others collect the resulting fluorescent signals. This would create a complete imaging system contained within the hair-thin profile.
Aside from brain surgery, the device could also help doctors perform more precise biopsies, identify cancer cells that are difficult to distinguish from healthy tissue, or monitor blood flow during delicate operations. In research settings, it could allow scientists to study how individual brain cells communicate in living animals without the tissue damage caused by current methods.
Current commercial systems, used to study brain activity in research animals, cost tens of thousands of dollars and provide only single-point measurements. The Microimager could offer 20 times more spatial information in a package that's dramatically less invasive.
With the ability to resolve features as small as 30 micrometers, this microscopic camera could successfully distinguish between different layers of brain tissue. Different cell types within these layers could be tagged with fluorescent proteins, allowing researchers to study their activity and explore connections within other brain regions.
This microscopic camera is giving a much needed upgrade to how we study and treat the most complex organ in the human body. Brain imaging could become less invasive and more detailed, accelerating our understanding of neurological diseases and improving outcomes for patients facing brain surgery.